RNA Library Kits/Modules

TIANSeq mRNA Capture Kit

Catalog number / packaging

Cat. no


No. of preps


NR105-01 24 rxn


NR105-02 96 rxn
  • Storage

    This kit can be stored at 4°C for 1 year.
  • Description

    TIANSeq mRNA Capture kit is designed to specifi cally capture messenger RNA (mRNA) by coupling the magnetic beads of Oligo (dT). The non-specific binding RNA can be removed by washing the beads with washing buff er, and the complete mRNA in total RNA of human, mouse, rat and plant can be obtained. The kit has a good mRNA capture effect for the complete total RNA. The obtained mRNA can be used for high-throughput sequencing, which can significantly improve the proportion of effective data in the sequencing results. In addition, the purified mRNA can also be used for random primer cDNA synthesis or other downstream applications.

  • Features

    ■ Wide range of samples: It is suitable for the capture of mRNA in high-quality (complete) samples. It is suggested that RIN of RNA should be more than 7.

    ■ Comprehensive data: Complete mRNA information is retained to improve the validity of transcriptome data.

    ■Wide range of application: Suitable for the capture of 100 ng-1 μg RNA.

  • Required Reagents

    Magnetic stand, rRNA primers, mRNA primers, qPCR quantification reagents.

  • Applications

    mRNA library construction and mRNA-Seq.
  • Table 1. Using TIANSeq mRNA capture Kit to capture the mRNA in the total RNA of human, mouse and rice with the input amount of 500 ng. RNA-Seq library was constructed by TIANSeq Fast RNA Library Kit (illumina), and the sequencing data analysis showed that the rRNA residue of the three types of samples were less than 2%,

    and the overall sequencing data quality was good.