● Wide range of samples: It is suitable for the capture of mRNA in high-quality (complete) samples. It is suggested that RIN (RNA Integrity Number) of RNA should be more than 7.

● Comprehensive data: Complete mRNA information is retained to improve the validity of transcriptome data.

● Wide range of application: Suitable for the capture of 100 ng-1 μg total RNA.

TIANSeq mRNA Capture kit is designed to specifically capture messenger RNA (mRNA) by coupling the magnetic beads of Oligo (dT). The non-specific binding RNA can be removed by washing the beads with washing buffer, and the complete mRNA in total RNA of human, mouse, rat and plant can be obtained. 

The kit has a good mRNA capture effect for the complete total RNA. The obtained mRNA can be used for high-throughput sequencing, which can significantly improve the proportion of effective data in the sequencing results. In addition, the purified mRNA can also be used for random primer cDNA synthesis or other downstream applications.

● mRNA capture: 8-Tube Strip (0.2 ml); Magnetic Frame (TIANGEN Cat# OSE-MF-04); Nuclease-Free centrifugation tube; Nuclease-Free PCR tube.

● PCR detection of mRNA capture efficiency: rRNA Primer Mix; mRNA Primer Mix; FastKing RT Kit (With gDNase) (TIANGEN Cat#4992223/4992224/4992250); SuperReal PreMix Plus (SYBR Green) (TIANGEN Cat#4992214/4992215/4992248).

● Please pay attention to avoid cross contamination between nucleic acid sample and product during operation.

● Please operate the experiment in the nucleic acid-free environment. The nucleic acid aerosol pollution in the operating environment should be removed in time.

● Please use DNase- and RNase-free pipette tips and centrifuge tubes for experiment.

● Before the experiment, please clean the operating table. It is recommended to use RNase and DNase cleaning reagent to treat the table surface. Make sure there is no contamination of RNase and DNase.