TIANSeq Size Selection DNA Beads is a product that can be used for DNA nucleic acid fragment sorting and purification. The product adopts high-performance magnetic beads and a unique buffer system. By changing the ratio of magnetic beads to sample,100 bp-1000 bp double-stranded and single-stranded DNA fragments can be sorted and purified without the need for electrophoresis separation and gel purification. The dNTP, salt ions and organic impurities

in the reaction system can be effectively removed by this kit, and the resulting DNA fragments are highly purified, with the recovery efficiency higher than 90%. This kit can be widely used for the sorting and purification of DNA fragments of specific length range in NGS library construction experiments.

Highly efficient: the DNA purification recovery efficiency can reach 90%.

Simple: No electrophoresis separation is required, while different sizes of DNA fragments ranging between 100bp-1000 bp can be sorted out directly by ratio adjustment.

Great compatibility: Compatible with high throughput operation in manual or automated workstations.

For DNA Fragment Sorting, refer toTable 1 for the recommended volumes for two rounds of sorting.

Table 1. Recommended MB Volume for DNA Fragment Sorting

TIANSeq Size Selection DNA Bead (4994217) was used to sort the PCR amplified library

according to the conditions in Table 1, to obtain libraries of different sizes. The figure

 shows theAgilent 2100 Bioanalyzer analysis of libraries of different sizes.

This kit can be widely applied to size-select and purify DNA fragments with specific lengths in NGS library construction.