TGuide FFPE DNA One-Step Kit is specially designed to extract genome DNA from paraffin tissue samples with TGuide automated nucleic acid extractors. This kit adopts a one-step heating method, so that deparaffinization and tissue lysis can be simultaneously carried out, and harmful reagents such as xylene are not contained. This kit has two extraction options: For small amount of samples, choose 2 hours lysis; For large amount of samples, choose 16 hours overnight lysis. The kit adopts cellulose embedded magnetic beads technology to efficiently extract genome DNA.

■ In-machine deparaffinization: The deparaffinization and extraction steps can be completed automatically by simply putting untreated paraffin embedded samples into reagent cartridge and then adding Sula Oil and Proteinase K.

■ Reliable results: The obtained genome DNA is free from RNA and protein contamination, and can be directly used for PCR or fluorescence real time PCR.

■ Safe and harmless: Organic solvents that are harmful to human body such as phenol chloroform are not needed.

Purified DNA can be directly used in various regular operations, including enzymatic digestion, PCR, library construction, Southern blot and other experiments.