Extraction Kits for Automatic Instruments
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Features
● Simple and fast: Ultra-pure total RNA can be obtained by running TGuide S16 for 59 minutes.
● Flexible throughput: It is a perfect fit for TIANGEN's TGuide S16 Nucleic Acid Extractor to extract 1~16 samples.
● Safe and non-toxic: No toxic reagents such as phenol/chloroform.
● High purity: The RNA obtained has high purity and can be directly used for chip detection, high-throughput sequencing and other experiments.
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Description
The kit adopts magnetic beads and a unique buffer system, to isolate and purify total RNA with high quality from blood/cell/tissue. The uniquely embedded magnetic beads have a strong affinity for nucleic acid under certain conditions. When the conditions are changed, the magnetic beads can release the absorbed nucleic acid to rapidly separate and purify it.
It can be used to perfectly fit with TGuide S16 Nucleic Acid Extractor. Through absorption, transfer and release of magnetic beads by the special magnetic bar, magnetic beads and nucleic acid can be transferred to improve the degree of automation. The whole process is safe and convenient, and the extracted RNA has high purity. If high throughput automated extraction is required, TIANGEN can deliver integrated solutions.
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Kit Contents
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Applications
RNA purified by this kit is suitable for downstream experiments including RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis and molecular cloning. -
Storage Condition
All components of the kit GDP661-E can be stored in dry conditions at room temperature (15~30°C) for 12 months. RNase-Free DNase I, Buffer RDD and RNase-Free ddH2O (in tubes) can be stored at 2~8°C for 15 months. -
Important Notes
● To use the TGuide Smart Blood/Cell/Tissue RNA Kit, you must have the TGuide Smart Blood/Cell/Tissue RNA (program no. DP661 and non-stop program DP661-01) installed on the TGuide S16/S32 Nucleic Acid Extractor.
● The product can be used with the TGuide S16 Nucleic Acid Extractor.
● DP661 program is suitable for all sample types. Pay attention to the pause step of DP661 (~30 mins after running). Users need to manually add 700 μl Buffer RD into the 3rd and 9th columns before the program can resume. DP661-01 is a non-stop program that is suitable for nucleic acid-rich samples. Users don't need to add Buffer RD manually when DP661-01 is chosen.
● Pay attention to the optimal storage and pre-processing conditions of samples to avoid degradation of extracted RNA. For sample stored in DNA and RNA stabilization solution, we recommend centrifuge to remove the storage buffer before sample pre-processing.
● During pre-processing of fresh blood samples, the customer is required to prepare 10 × Red cell lysis buffer (not provided) and RNase-Free Columns CS (TIANGEN, GRK176, not provided).
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Before the First Use
● Prepare DNase I stock solution: Dissolve the lyophilized DNase I (1500 units) in 550 μl of the RNase-Free ddH2O. Do not remove the rubber top of the vial to prevent loss of DNase I powder. Use a syringe and a needle to inject the RNase-Free ddH2O into the vial. Mix gently by inverting. Do not vortex. Divide the solution into single-use aliquots, and store at -30~-15°C for up to 9 months. Thawed aliquots can be stored at 2~8℃ for up to 6 weeks. Do not refreeze the aliquots after thawing.
● Add absolute ethanol into Buffer RD according to the volume stated on the label of the bottle.
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Reagents Composition
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Program
● The extraction process of S16 provided for DP661 is shown in the following table:This program is suitable for all sample types.
● The extraction process of S16 provided for non-stop DP661-01 is shown in the following table. DP661-01 is suitable for nucleic acid rich samples.
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Related Products
● Instrument and Accessories
● TGuide Smart Reagent Kits
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