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Extraction Kits for Automatic Instruments

TGuide Smart Blood/Cell/Tissue RNA Kit

The prefilled cartridge / plate reagent kit for purification of high-yield, high-purity, high-quality, inhibitor-free total RNA from animal tissue/cell/fresh whole blood

Catalog number / packaging

Cat. no

ID

No. of preps

4995043(plate)

 DP661-E 96

4993551(cartridge)

 DP661-DE 48
Manual Inquire Inspection report

  • Reagent selection guide for TGuide S16



    Nuclei Acid Sample Type Product Name

    Packing size (preps)

    Cartridge/plate

    Cat no.

    Cartridge/plate

    DNA Animal Tissue
    		 TGuide Smart Magnetic Tissue DNA Kit 48/96
    		 4993547/4995038
    DNA Plant & Seed TGuide Smart Magnetic Plant DNA Kit 48 4993548
    DNA Soil/Stool TGuide Smart Soil /Stool DNA Kit 48 4993549
    DNA Gel & PCR products TGuide Smart DNA Purification Kit 48 4993550
    DNA  Whole Blood TGuide Smart Blood Genomic DNA Kit 48/96
    		 4993703/4995206
    DNA Cell/Swab/Dry Spots,etc TGuide Smart Universal DNA Kit 48/96
    		 4993704/4995040
    RNA Blood/Cell/Tissue TGuide Smart Blood/Cell/Tissue RNA Kit 48/96
    		 4993551/4995043
    RNA Plant & Seed TGuide Smart Magnetic Plant RNA Kit 48 4993552
    DNA/RNA Cell Free Fluids (Serum,etc) TGuide Smart Viral DNA/RNA Kit 48/96
    		 4993702/4995207


  • Product introduction

    •Purify high-yield, high-purity, high-quality total RNA from blood/cell/tissue.

    •No toxic reagents such as phenol/chloroform contained

    •The RNA obtained has high purity and can be directly used for chip detection, high-throughput sequencing and other experiments.

  • Features

    Super easy to use


    No extra pipetting work.

    The elution volume can be adjusted as required.



    The installation and operation

    require little training. With pre-installed programs, unpack the cartridge, select the protocol and run your experiment. Programs are ready-to-use, and also customizable.


    Preloaded reagents and matched disposable consumables

    The chemical handling is eliminated to the greatest extent to ensure the operation safety.



  • Agilent 2100 was used for integrity detection.

    Sample source: CD-1 mice

    Sample status: fresh whole blood

    Sample size: 200 μl

    Experiment result: the total RNA extracted from blood by TGuide S16 equipped with reagent 4993551 has good yield and high purity, and can meet the needs of downstream

    high-throughput sequencing.

  • Sample source: CD-1 mice

    Loading volume: 1 μl. The liver was diluted 5 times and loaded.

    Agarose gel concentration: 1%. Electrophoresis for 20 min at 6 V/cm;

    M:TIANGEN Marker III   1-4: heart 5-8:

    liver 9-12: lung 13-16: kidney

    The first two samples of each tissue were extracted by the spin-column based extraction kit, and the last two were extracted automatically by TGuide S16.

  • Experiment result: the animal tissue total RNA automatically extracted by TGuide S16 equipped with reagent 4993551 has high yield and purity, which is equivalent to the RNA extracted by the spin-column based extraction protocol. The purity is basically the same, with OD260/OD280 around 2.0~2.1 and OD260/OD230 > 2.0. In conclusion, TGuide S16 can be used to extract the total RNA from animal tissues in replacement of spin-column based extraction solution.

Publication

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