Nucleic Acid Extraction Reagent
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Description
Red Cell Lysis Buffer contains ammonium chloride, which is designed for the preferential lysis of red blood cells from human whole blood, yielding intact white blood cells (free of red blood cells) for further applications.
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Storage Condition
Stored at room temperature (15-30°C) for 24 months. -
Protocol
● Add three volume Red Cell Lysis Buffer to blood sample (e.g. add 900 μl Red Cell Lysis Buffer to 300 μl blood), mix completely by inverting the tube upside down.
● Incubate for 5 min at room temperature, and invert upside down during that time.
● Centrifuge for 1 min at 10,000 rpm (if it’s unavailable for centrifuge, then please apply 5 min at 3,000 rpm).
● Remove the supernatant, and leave white cells for following experiment.
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