Nucleic Acid Extraction Reagent
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Features
● RNasin efficiently inhibits the activity of eukaryotic RNaseA, RNaseB, RNaseC and human placenta RNase.
● RNasin is compatible with enzymes including RNase H, nuclease S1, RNA polymerase SP6, T7, T3, reverse transcriptase AMV or M-MLV, Taq DNA polymerase, RNase T1. It has no influence upon the transcription and translation processes.
● RNasin has wide pH activity ranges. (1 mM DTT in the buffer system is needed.)
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Description
RNasin is a broad spectrum ribonuclease inhibitor expressed by recombinant E.coli. It is essentially a protein with molecular weight of 50 kDa and used very generally in molecular biology experiments such as RT-PCR, protecting mRNA in cDNA synthesis. In vitro transcription and in vitro translation, preparing RNase-Free antibody, in situ hybridization, mRNA positioning, etc. It is a useful reagent in any application against potential RNase.
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Applications
● Where there is potential RNase contamination.
● To protect mRNA in cDNA synthesis, in vitro transcription and in vitro translation.
● Can increase the activity and yield of multiple ribosomes and be beneficial to the replication of virus in vitro.
● To prepare protein products without RNase such as antibody.
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Storage Condition
-30~-15°C for up to 18 months. -
Instruction
According to the definition that 50% of 5 ng RNase A activity is inhibited by one unit of enzyme, RNasin can be added at a final concentration of 5 U/μl. Please adjust the dosage according to the specific conditions of the experiment. Usually, 0.5-1 μl (about 20-40 U) of RNasin is added to a 20 μl reverse transcription system. -
Protocol
Add the RNasin to the samples to a final concentration of 5 U/μl or optimized the amount for individual experiment.
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