Nucleic Acid Extraction Reagent
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Storage
Store at -20℃ -
Description
RNasin is a specific ribozyme (RNase) inhibitor found in human placenta. It is a protein with the molecular weight 51,000 Da, and the isoelectric pH value 4.7. RNasin can specifically bind to RNase via non-covalent bond to form a complex that inactivates RNase. RNasin is active in the buffer condition of 0.5 M NaCl, pH 5-8, and has the highest activity at pH 7.8. RNasin protects the integrity of mRNA and improves the efficiency of transcription and translation, while avoiding the possible effects of using organic compound inhibitors. -
Instruction
According to the definition that 50% of 5 ng RNase A activity is inhibited by one unit of enzyme, RNasin can be added at a final concentration of 5 U/μl. Please adjust the dosage according to the specific conditions of the experiment. Usually, 0.5-1 μl (about 20-40 U) of RNasin is added to a 20 μl reverse transcription system. -
Features
■ RNasin inhibits the activity of RNaseA, B, C in eukaryotes and human placental RNase.
■ This product does not inhibit RNase H, S1 nuclease, SP6, T7 and T3 RNA polymerase, MV or M-MLV reverse transcriptase, Taq DNA polymerase, RNase T1, and does not affect subsequent reverse transcription and protein translation process.
■ The buffer system requires 1 mM DTT. The pH range for active RNasin is wide.
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Applications
■ Can be used in areas with potential RNase contamination.
■ Protection of mRNA during cDNA synthesis, in vitro transcription systems, and translation systems.
■ Increase the activity and yield of polysomes and facilitate the in vitro replication of the virus.
■ Can be used to prepare RNase-free protein products, such as antibodies.
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