Electrophoresis Reagent

GeneGreen Nucleic Acid Dye

Safe, nontoxic and highly sensitive EB substitute dye

Catalog number / packaging

Cat. no

ID

No. of preps

4992961

RT210 500 µl
  • Storage

    Store at 2~8°C in dark place in dry condition for 12 months
  • Description

    GeneGreen Nucleic Acid Dye is an improved oily macromolecule based on cyanine, which reduces the influence of traditional cyanine dyes on the mobility of nucleic acids during electrophoresis, and does not cause electrophoresis band bending. The dye is not volatile and cannot penetrate cell membrane into living cells or be inhaled into human body. The mutagenicity is far lower than EB. It can be cut and recycled under the blue visible light excitation device, which is safe and convenient. It is a new nucleic acid dye with safety, innocuity and high sensitivity.


  • Features

    ■ Safe and non-toxic: The unique characteristic of oily macromolecules prevents it from penetrating cell membrane and entering the cells. The mutagenicity of this dye is far lower than EB.

    ■ Perfect compatibility: Suitable for 254 nm UV gel imaging system and blue visible light excitation device. When the gel cutting operation is carried out under blue light, the damage to eyes and skin of operators caused by UV gel cutting and damage to nucleic acid products caused by long-term exposure to UV light can be avoided.

    ■ High sensitivity: Strong fluorescence signal, low background signal and high signal-to-noise ratio.



  • Effect of GeneGreen Nucleic Acid Dye Combined with OSE-470

  • Image of GeneGreen Dye under UV light



    Dilute TIANGEN Marker III with 2-fold gradient to a total of 6 gradients including the original concentration. 6 μl DNA was loaded, and the brightest band (1200 bp) was 100 ng, 50 ng, 25 ng, 12.5 ng, 6.25 ng, 3.125 ng in sequence.

    Conclusion: GeneGreen has the same sensitivity as EB under UV imaging, but GeneGreen has higher sensitivity under blue-light than EB under UV imaging. Comparing to EB Dye, GeneGreen is more flexible and convenient to use.


  • Image of GeneGreen Dye under blue-light


    Dilute TIANGEN Marker III with 2-fold gradient to a total of 6 gradients including the original concentration. 6 μl DNA was loaded, and the brightest band (1200 bp) was 100 ng, 50 ng, 25 ng, 12.5 ng, 6.25 ng, 3.125 ng in sequence. 

    Conclusion: GeneGreen has the same sensitivity as EB under UV imaging, but GeneGreen has higher sensitivity under blue-light than EB under UV imaging. Comparing to EB Dye, GeneGreen is more flexible and convenient to use.


  • Image of EB Dye under UV light

    Dilute TIANGEN Marker III with 2-fold gradient to a total of 6 gradients including the original concentration. 6 μl DNA was loaded, and the brightest band (1200 bp) was 100 ng, 50 ng, 25 ng, 12.5 ng, 6.25 ng, 3.125 ng in sequence.

    Conclusion: GeneGreen has the same sensitivity as EB under UV imaging, but GeneGreen has higher sensitivity under blue-light than EB under UV imaging. Comparing to EB Dye, GeneGreen is more flexible and convenient to use.