Electrophoresis Reagent

GeneRed Nucleic Acid Dye

Highly sensitive safety dyes

Catalog number / packaging

Cat. no

ID

No. of preps

4992962

RT211 500 µl
  • Storage

    Store at 2~8°C in dark place in dry condition for 12 months
  • Description

    GeneRed Nucleic Acid Dye is a safe nucleic acid dye of EB substitute. It's an oily macromolecule that is not easy to volatilize and cannot penetrate cell membrane into cells. The mutagenicity of the dye is far less than EB. The product is suitable for electrophoretic dyeing of fragments of various sizes. With less influence on nucleic acid migration

    and extremely high thermal stability, it is suitable for preparing agarose gel by microwave or other heating methods.


  • Features

    ■ Safe and nontoxic: The unique oily macromolecule is not easy to volatilize and cannot penetrate cell membrane into cells. The mutagenicity of the dye is far less than EB.

    ■ High sensitivity: Suitable for electrophoresis staining of fragments of various sizes, with little influence on nucleic acid migration.

    ■ High stability: Suitable for preparing agarose gel by microwave or other heating methods; Extremely stable in acid or alkali buffer at room temperature and has strong light resistance.

    ■ High signal-to-noise ratio: The sample has strong fluorescence signal and low background signal.

    ■ Simple operation: It will not degrade in the process of gel preparation and electrophoresis. After electrophoresis dyeing, decolorization or washing are not needed and it can be directly observed by UV transilluminator.

    ■ Wide application: Dyeing before electrophoresis (gel dyeing method) or dyeing after electrophoresis (soaking and dyeing) is optional; Suitable for agarose gel or polyacrylamide gel electrophoresis; Applicable for dsDNA, ssDNA or RNA staining.

    ■ It has the same spectral characteristics as EB and does not need to change filters and observation devices. The standard EB filter or SYBR filter are applicable. It can be observed by a common UV transilluminator, and the best excitation can be obtained near 300 nm ultraviolet light.




  • EB Dyeing Results

    D2000 samples (100 ng, 50 ng, 25 ng, 12.5 ng, 6.25 ng, 3.125 ng) were used for the comparison of dyes (EB, GeneRed). Run in 1% agarose gel, 160V, 25min

  • GeneRed Dyeing Results

    D2000 samples (100 ng, 50 ng, 25 ng, 12.5 ng, 6.25 ng, 3.125 ng) were used for the comparison of dyes (EB, GeneRed). Run in 1% agarose gel, 160V, 25min