Phase Lock GelTM (PLG) is a proprietary tool for avoiding contamination of the protein layer when extracting DNA or RNA with organic reagents. PLG shortens the extraction operation time while increasing the nucleic acid yield. Under the action of centrifuge, PLG can form a dense stationary phase between the water phase and the organic phase, and the substances in the organic phase are effectively isolated below PLG. The formation of a dense solid-phase layer allows the experimenter to easily transfer nucleic acids from the aqueous phase into clean tubes.

When PLG is used in nucleic acid extraction experiment, the yield of nucleic acid can be increased by 10%-20%, which can effectively avoid the contact between experimenters and toxic substances, and there is no need to worry about whether the sample will be contaminated when extracting. PLG can be applied to the experimental operation of liquid phase extraction with any organic reagent (phenol or chloroform).

PLG has two densities, Heavy (H) and Light (L), which are suitable for any pure phenol, phenol/chloroform and chloroform extractions. The former is suitable for the extraction of plasmids, RNA and other samples containing high impurities (viscous) (not suitable for pure phenol extraction). The latter is suitable for restriction enzymatic digestion, cDNA synthesis, labeling reaction and conventional tissue gene DNA extraction (except for rat tail). The methods can also be selected by the compatibility formula of aqueous phase and organic phase, as listed in the following table.