-30~-15°C for 2 years. For frequently use, it can be stored at 2-8°C for 3 months.

Marker D2000 consists of six double-stranded DNA bands that are suitable as molecular weight standards for agarose gel electrophoresis.

The product is ready-to-use; it is premixed with 1× Loading Buffer for direct loading 3~6 μl on agarose gel electrophoresis.

The ladder is composed of six individual DNA fragments (in base pairs): 2000, 1000, 750, 500, 250, and 100. It contains one intense band (750 bp) for easy orientation. 

To load 6 μl of the product will provide 50 ng per DNA fragment with one intense fragment of 100 ng.

10 mM Tris-HCl (pH 8.4)

10 mM EDTA

0.02% Bromophenol Blue

5% Glycerol

● Directly load 3~6 μl of the product on the agarose gel (load 1 μl per 1 mm gel lane. The loading volume can be increased appropriately for wide lanes).

● Electrophoresis condition: 1.0~2.5% agarose gel, 4~10v/cm voltage.

● DNA marker can be visualized using ethidium bromide under UV light.

● Directly use, do not heat before loading.

● Please use new agarose gel and change electrophoresis buffer in time, so as not to affect the electrophoresis results.