DNA Marker

D2000

Catalog number / packaging

Mat. No

Ref. No

No. of preps

4992949

GMD114-01 300 µl (50 lanes)

4992950

GMD114-02 4 × 300 µl (200 lanes)
  • Storage Condition

    -30~-15°C for 2 years. For frequently use, it can be stored at 2-8°C for 3 months.


  • Concentration

    About 0.05 mg DNA/ml
  • Description

    Marker D2000 consists of six double-stranded DNA bands that are suitable as molecular weight standards for agarose gel electrophoresis.

    The product is ready-to-use; it is premixed with 1× Loading Buffer for direct loading 3~6 μl on agarose gel electrophoresis.

    The ladder is composed of six individual DNA fragments (in base pairs): 2000, 1000, 750, 500, 250, and 100. It contains one intense band (750 bp) for easy orientation. 

    To load 6 μl of the product will provide 50 ng per DNA fragment with one intense fragment of 100 ng.


  • Buffer Contents

    10 mM Tris-HCl (pH 8.4)

    10 mM EDTA

    0.02% Bromophenol Blue

    5% Glycerol

  • Protocol

    ● Directly load 3~6 μl of the product on the agarose gel (load 1 μl per 1 mm gel lane. The loading volume can be increased appropriately for wide lanes).

    ● Electrophoresis condition: 1.0~2.5% agarose gel, 4~10v/cm voltage.

    ● DNA marker can be visualized using ethidium bromide under UV light.

  • Recommendations

    ● Directly use, do not heat before loading.

    ● Please use new agarose gel and change electrophoresis buffer in time, so as not to affect the electrophoresis results.

Sort by

Date Date()

Date Date()

Impact Factor IF()

Impact Factor IF()