The 100 bp DNA Ladder marker consists of eleven double-stranded DNA bands that are suitable as molecular weight standards for agarose gel electrophoresis.

Since this product is premixed with 1× Loading Buffer, it is convenient to load 3~6 μl of this product to agarose gel electrophoresis directly.

The ladder is composed of eleven individual DNA fragments (in base pairs): 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200, and 100. It contains one intense band (500 bp) for easy orientation. To load 6 μl of the product will provide 50 ng per DNA fragment with one intense fragment of 100 ng.

-30~-15°C for 2 years. For frequently use, it can be stored at 2-8°C for 3 months.

10 mM Tris-HCl (pH 8.4)

10 mM EDTA

0.02% Bromophenol Blue

5% Glycero

● Directly load 3~6 μl of this product to the agarose gel (load 1μl product per 1 mm gel lane. The loading volume can be increased appropriately for wide lanes).

● Electrophoresis condition: 0.5~1.0% agarose gel, 4~10 v/cm voltage.

● DNA marker can be visualized using ethidium bromide under UV light.

● Directly use, do not require heating.

● For ideal results, please use new agarose gel and change electrophoresis buffer in time.