Novel High-Fidelity PCR Master Mix

Simple, Practical, Stable, and Durable

One-hand operation, extremely accurate

Room temperature gel dissolution, fast and high efficient gel recovery

One kit, two functions. Monitoring the solution colors makes the purification simple.

Safe and convenient gel observation/cutting assistant

An innovative all-in-one smart solution for low throughput nucleic acid extraction experiments

An innovative all-in-one smart solution for low throughput nucleic acid extraction experiments

The EasyGeno Single-Fragment Recombinational Cloning Kit enables rapid directional cloning of a single DNA fragment into any linearized vector, based on sequence homology between the fragment and vector ends. Through meticulous design and rigorous testing, the included 2× EasyGeno Single Assembly Mix contains enzymatic activity that mediates recombination and linkage between the homologous regions of the vector and the insert starting from the 3′ ends of the double-stranded DNA. This results in a seamless closed-circular plasmid that can be directly transformed into competent cells for clone selection. This method overcomes the limitations of restriction enzyme sites: only the terminal sequences of the linearized vector need to be introduced at the 5′ ends of the PCR primers used to amplify the insert. This generates 15–25 bp overlaps between the insert ends and the corresponding vector ends, allowing directional recombination of the fragment into the vector. The recombination reaction is performed rapidly under isothermal conditions (50 ℃ for 15 minutes). Combined with flexible PCR product handling options and a rapid transformation protocol, the entire process—from DNA sample preparation to transformation and plating—can be completed in just a few hours.

The EasyGeno Assembly Cloning Kit enables rapid directional cloning of single or multiple DNA fragments into any linearized vector, based on sequence homology between the fragments and the vector. The thoughtfully formulated one-tube master mix—2× EasyGeno Assembly Mix—facilitates recombination and ligation between the linearized vector and insert fragments at homologous regions, producing seamless closed-circular plasmids. These can be directly transformed into competent cells for clone selection. This method eliminates dependence on restriction enzyme sites: directional recombination of the target fragment into the vector is achieved simply by incorporating 15 bp homologous ends of the linearized vector onto both ends of the insert via PCR. The recombination reaction is performed rapidly under isothermal conditions (50 ℃ for 15 minutes), with a positive clone rate exceeding 95%. In addition to single-fragment cloning, the kit also supports simultaneous directional assembly of multiple fragments into a vector, enabling high-throughput construct generation

EasyGeno Primer EasyGeno Primer is a specialized primer design tool developed for use with the EasyGeno Rapid Recombinational Cloning Kit (VI201). The software requires only the vector sequence, the sequence and number of DNA insert(s), and the method of vector linearization to generate PCR primers for introducing overlapping sequences. It offers both Chinese and English versions to accommodate users of different languages.

Highly efficient experimental assistant with all in one rotor design

Perfect performance for temperature control experiment

An innovative all-in-one smart solution for low throughput nucleic acid extraction experiments

Highly efficient experimental assistant with all in one rotor design

Efficient and Highly Tolerant PCR Master Mix for Robust Amplification

Efficient and Highly Tolerant PCR Master Mix for Robust Amplification

SSimple, Practical, Stable, and Durable

One-hand operation, extremely accurate

An innovative all-in-one smart solution for low throughput nucleic acid extraction experiments

Purification of high quality plasmid DNA by advanced silica membrane based technique

For fast purification of plasmid DNA of molecular biology grade by alkaline lysis technique

For purification of transfection grade plasmid DNA

For extraction ultralow endotoxin plasmid from 20-50 ml bacteria culture

Purification of endotoxin-free transfection grade plasmid DNA specific for sensitive cells

Perfect performance for temperature control experiment